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Cellosaurus A-375 Human EGFR vIII (CVCL_XZ66)

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Cell line name A-375 Human EGFR vIII
Synonyms A375-EGFRVIII; U87MG Human EGFR vIII
Accession CVCL_XZ66
Resource Identification Initiative To cite this cell line use: A-375 Human EGFR vIII (RRID:CVCL_XZ66)
Comments Problematic cell line: Misidentified. Was originally thought to originate from the U87MG cell line. STR profiling carried out at KYinno has shown it was derived from A-375. Be aware of this issue if you obtained this cell line before it was indicated as being derived from K-562.
Population: Caucasian.
Doubling time: ~30 hours (KYinno=KC-1308).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3236; EGFR (Note=With p.Val30_Arg297del = EGFRvIII).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple_edited; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous; Note=By method not specified (Horizon_Discovery=HD+118-001).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0132 (A-375)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) KYinno; KC-1308
Cell line databases/resources cancercelllines; CVCL_XZ66
Encyclopedic resources Wikidata; Q98133791
Entry history
Entry creation19-Dec-2019
Last entry update14-Aug-2025
Version number11