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Cellosaurus HepaRG-CAR (CVCL_XI49)

[Text version]
Cell line name HepaRG-CAR
Accession CVCL_XI49
Resource Identification Initiative To cite this cell line use: HepaRG-CAR (RRID:CVCL_XI49)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ); ACC-3291.
Characteristics: Compared to the parent cell line, has improved liver functionality. Drug metabolism is highly improved. Culturing can be performed in serum-free conditions both in the presence or absence of DMSO.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7969; NR1I3.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Disease Hepatitis C infection (NCIt: C3098)
Hepatocellular carcinoma (NCIt: C3099)
Hepatocellular carcinoma (ORDO: Orphanet_88673)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9720 (HepaRG)
Sex of cell Female
Age at sampling Age unspecified
Category Cancer cell line
Publications

PubMed=27780834; DOI=10.1124/dmd.116.072603
Vincent A. van der Mark, Dirk Rudi de Waart, Valery Shevchenko, Ronald P.J. Oude Elferink, Robert A.F.M. Chamuleau, Ruurdtje Hoekstra;
Stable overexpression of the constitutive androstane receptor reduces the requirement for culture with dimethyl sulfoxide for high drug metabolism in HepaRG cells.
Drug Metab. Dispos. 45:56-67(2017)

Patent=US20190256822
Vincent A. van der Mark;
Hepatic cell line resistant to dimethyl sulfoxide, cell culture and uses thereof.
Patent number US20190256822, 22-Aug-2019

PubMed=32130581; DOI=10.1007/s10616-020-00384-w; PMCID=PMC7225227
Aziza A.A. Adam, Aldo Jongejan, Perry D. Moerland, Vincent A. van der Mark, Ronald P.J. Oude Elferink, Robert A.F.M. Chamuleau, Ruurdtje Hoekstra;
Genome-wide expression profiling reveals increased stability and mitochondrial energy metabolism of the human liver cell line HepaRG-CAR.
Cytotechnology 72:377-395(2020)

Cross-references
Encyclopedic resources Wikidata; Q94103091
Entry history
Entry creation19-Dec-2019
Last entry update14-Aug-2025
Version number12