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Cellosaurus SK-HEP-1-Cas9-731 (CVCL_XD89)

[Text version]
Cell line name SK-HEP-1-Cas9-731
Accession CVCL_XD89
Resource Identification Initiative To cite this cell line use: SK-HEP-1-Cas9-731 (RRID:CVCL_XD89)
Comments Problematic cell line: Misclassified. Parent cell line (SK-HEP-1) was originally described as originating from an adenocarcinoma of liver and thus classified as hepatocellular carcinoma. Later studies show that it most probably has arisen from endothelial cells.
Population: Caucasian.
Characteristics: Transduced with a Cas9-FLAG-PuroR construct.
Karyotypic information: Has lost chromosome Y (from parent cell line).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a C-terminal FLAG tag).
Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
Cell type: Endothelial cell; CL=CL_0000115.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
Disease Liver and intrahepatic bile duct epithelial neoplasm (NCIt: C7106)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0525 (SK-HEP-1)
Sex of cell Male
Age at sampling 52Y
Category Cancer cell line
STR profile Source(s): CCRID=1101HUM-PUMC000731

Markers:
AmelogeninX
CSF1PO11,12
D2S133820,23
D3S135816
D5S81810,13
D6S104311
D7S8208,11
D8S117913,14
D12S39118
D13S3178,12
D16S53912
D18S5113,15
D19S43312,15.2
D21S1129,31,32
FGA17
Penta E13,21
TH017,9
TPOX9
vWA14,17

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Cross-references
Cell line databases/resources cancercelllines; CVCL_XD89
CCRID; 1101HUM-PUMC000731
Encyclopedic resources Wikidata; Q98132650
Entry history
Entry creation06-Sep-2019
Last entry update10-Apr-2025
Version number15