Cellosaurus cell line PLC/PRF/5-Cas9-708 (CVCL

Cellosaurus PLC/PRF/5-Cas9-708 (CVCL_XB35)

Cross-references
Cell line name	PLC/PRF/5-Cas9-708
Accession	CVCL_XB35
Resource Identification Initiative	To cite this cell line use: PLC/PRF/5-Cas9-708 (RRID:CVCL_XB35)
Comments	Population: African; Shangaan. Characteristics: Transduced with a Cas9-FLAG-Neo construct. Karyotypic information: Has lost chromosome Y (from parent cell line). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a C-terminal FLAG tag). Transformant: NCBI_TaxID; 10407; Hepatitis B virus (HBV). Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations	Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg112Gly (c.334C>G) (p.Pro126Arg, c.377C>G); ClinVar=VCV000233484; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg249Ser (c.747G>T); ClinVar=VCV000012352; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:12363; TSC2; Simple; p.Asn1651Ser (c.4952A>G); ClinVar=VCV000049335; Zygosity=Homozygous (from parent cell line).
Disease	Adult hepatocellular carcinoma (NCIt: C7956) Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0485 (PLC/PRF/5)
Sex of cell	Male
Age at sampling	24Y
Category	Cancer cell line
Cell line databases/resources	cancercelllines; CVCL_XB35 CCRID; 1101HUM-PUMC000708
Encyclopedic resources	Wikidata; Q98128623
Entry history
Entry creation	06-Sep-2019
Last entry update	14-Aug-2025
Version number	13