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Cellosaurus WAe009-A-23 (CVCL_WZ23)

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Cell line name WAe009-A-23
Synonyms PITX3-mCherry hESC; WAe009-A-PITX3; YONSEIe010-A-23
Accession CVCL_WZ23
Resource Identification Initiative To cite this cell line use: WAe009-A-23 (RRID:CVCL_WZ23)
Comments From: Yonsei University College of Medicine; Seoul; South Korea.
Characteristics: Using CRISPR/Cas9 a T2A-mCherry-P2A-PGKp-Neo-P2A construct was integrated in front of the stop codon of PITX3.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=31352199; DOI=10.1016/j.scr.2019.101499
Sanghyun Park, Jeong-Eun Yoo, Dongjin R. Lee, Jiho Jang, Myung Soo Cho, Dae-Sung Kim, Dong-Wook Kim;
Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23).
Stem Cell Res. 39:101499-101499(2019)

Cross-references
Cell line databases/resources hPSCreg; WAe009-A-23
SKIP; SKIP005556
Encyclopedic resources Wikidata; Q98134746
Entry history
Entry creation06-Sep-2019
Last entry update14-Aug-2025
Version number7