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Cellosaurus CHO-pEDdc-hPRL (CVCL_WS57)

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Cell line name CHO-pEDdc-hPRL
Accession CVCL_WS57
Resource Identification Initiative To cite this cell line use: CHO-pEDdc-hPRL (RRID:CVCL_WS57)
Comments Characteristics: The sequence of PRL was inserted in the pEDdc construct (PubMed=11001873).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:9445; PRL.
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_1977 (CHO-DXB11)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=11001873; DOI=10.1042/BA20000047
Carlos Roberto Jorge Soares, Ligia Morganti, Brigitte Miloux, Johannes Hermanus Lupker, Pascual Ferrara, Paolo Bartolini;
High-level synthesis of human prolactin in Chinese-hamster ovary cells.
Biotechnol. Appl. Biochem. 32:127-135(2000)

PubMed=22692846; DOI=10.1007/s12010-012-9745-1
Fernanda Santos Arthuso, Paolo Bartolini, Carlos Roberto Jorge Soares;
Laboratory production of human prolactin from CHO cells adapted to serum-free suspension culture.
Appl. Biochem. Biotechnol. 167:2212-2224(2012)

Cross-references
Cell line databases/resources FCS-free; 24-8-406-3-4-12
Encyclopedic resources Wikidata; Q93452607
Entry history
Entry creation05-Jul-2019
Last entry update19-Dec-2024
Version number8