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Cellosaurus R1-Oct4-EGFP (CVCL_WS52)

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Cell line name R1-Oct4-EGFP
Accession CVCL_WS52
Resource Identification Initiative To cite this cell line use: R1-Oct4-EGFP (RRID:CVCL_WS52)
Comments Characteristics: Transfected with a construct containing Pou5f1/Oct4-eGFP and PGKp-neo-pA.
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=MGI; MGI:101893; Pou5f1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: 129X1/SvJ x 129S1/Sv-Oca2+Tyr+KitlSl-J.
Hierarchy Parent: CVCL_2167 (ES-R1)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=22714273; DOI=10.1007/s10529-012-0973-9
Li Liu, Qing-Hua Yuan, Jian Shi, Xin Li, Dong-Ju Jung, Li Wang, Kaori Yamauchi, Norio Nakatsuji, Ken-ichiro Kamei, Yong Chen;
Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions.
Biotechnol. Lett. 34:1951-1957(2012)

Cross-references
Cell line databases/resources FCS-free; 102-5-153-2-4-3
Encyclopedic resources Wikidata; Q98128776
Entry history
Entry creation05-Jul-2019
Last entry update14-Aug-2025
Version number8