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Cellosaurus SpESFX-2 (CVCL_WS51)

[Text version]
Cell line name SpESFX-2
Synonyms SpESF-X2; SpESFX clone 2
Accession CVCL_WS51
Resource Identification Initiative To cite this cell line use: SpESFX-2 (RRID:CVCL_WS51)
Comments Group: Hybridoma fusion partner cell line.
Group: Patented cell line.
Group: Serum/protein free medium cell line.
Characteristics: Transfected with a hBCL2-EEE-pZeoSV2+ construct.
Selected for resistance to: ChEBI; CHEBI_63486; 8-azaguanine.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:990; BCL2 (Note=With p.Thr69Glu, p.Ser70Glu and p.Ser87Glu = BCL2-EEE).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Cell type: Plasma cell; CL=CL_0000786.
Disease Mouse multiple myeloma (NCIt: C125417)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_WS49 (SpESFX)
Sex of cell Female
Age at sampling Age unspecified
Category Cancer cell line
Publications

Patent=US7608425
David M. Goldenberg, Chien-Hsing Chang, Edmund A. Rossi, Diane Nordstrom;
Methods for protein expression in mammalian cells in serum-free medium.
Patent number US7608425, 27-Oct-2009

Cross-references
Encyclopedic resources Wikidata; Q98132817
Entry history
Entry creation05-Jul-2019
Last entry update14-Aug-2025
Version number8