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Cellosaurus SpESFX (CVCL_WS49)

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Cell line name SpESFX
Synonyms SpESF-X
Accession CVCL_WS49
Resource Identification Initiative To cite this cell line use: SpESFX (RRID:CVCL_WS49)
Comments Group: Hybridoma fusion partner cell line.
Group: Patented cell line.
Group: Serum/protein free medium cell line.
Characteristics: Transfected with a hBCL2-EEE-pZeoSV2+ construct.
Selected for resistance to: ChEBI; CHEBI_63486; 8-azaguanine.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:990; BCL2 (Note=With p.Thr69Glu, p.Ser70Glu and p.Ser87Glu = BCL2-EEE).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Cell type: Plasma cell; CL=CL_0000786.
Disease Mouse multiple myeloma (NCIt: C125417)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_WS48 (SpESF)
Children:
CVCL_WS50 (SpESFX-10)CVCL_WS51 (SpESFX-2)
Sex of cell Female
Age at sampling Age unspecified
Category Cancer cell line
Publications

Patent=US7608425
David M. Goldenberg, Chien-Hsing Chang, Edmund A. Rossi, Diane Nordstrom;
Methods for protein expression in mammalian cells in serum-free medium.
Patent number US7608425, 27-Oct-2009

PubMed=21473000; DOI=10.1002/btpr.584
Diane L. Rossi, Edmund A. Rossi, David M. Goldenberg, Chien-Hsing Chang;
A new mammalian host cell with enhanced survival enables completely serum-free development of high-level protein production cell lines.
Biotechnol. Prog. 27:766-775(2011)

Cross-references
Encyclopedic resources Wikidata; Q98132815
Entry history
Entry creation05-Jul-2019
Last entry update14-Aug-2025
Version number8