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Cellosaurus C17 NKX2-1-GFP SFTPC-tdTomato (CVCL_WN83)

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Cell line name C17 NKX2-1-GFP SFTPC-tdTomato
Synonyms C17 NKX2-1GFP;SFTPCtdTomato; C17-NGST; C17 NGST; iPS17-ST
Accession CVCL_WN83
Resource Identification Initiative To cite this cell line use: C17 NKX2-1-GFP SFTPC-tdTomato (RRID:CVCL_WN83)
Comments From: Boston University School of Medicine; Boston; USA.
Population: Caucasian.
Characteristics: Using TALEN a eGFP-pA-loxP-PGKp-PuroR-pA-loxP was inserted at the NKX2-1 translational start site and a tdTomato-pA-loxP-PGKp-PuroR-pA-loxP was inserted at the SFTPC translational start site.
Genetic integration: Method=TALEN; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Cell type: Fibroblast of skin; CL=CL_0002620.
Sequence variations
  • Mutation; HGNC; HGNC:1884; CFTR; Simple; p.Ile507del (c.1516_1518ATC[1]) (c.1519_1521delATC); ClinVar=VCV000007106; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1884; CFTR; Simple; p.Phe508del (c.1521_1523delCTT); ClinVar=VCV000007105; Zygosity=Heterozygous (from parent cell line).
Disease Cystic fibrosis (NCIt: C2975)
Cystic fibrosis (ORDO: Orphanet_586)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D3D6 (C17 NKX2-1-GFP)
Sex of cell Male
Age at sampling 17Y
Category Induced pluripotent stem cell
Publications

PubMed=28965766; DOI=10.1016/j.stem.2017.08.014; PMCID=PMC5755620
Anjali Jacob, Michael Morley, Finn Hawkins, Katherine B. McCauley, Jyh-Chang Jean, Hillary Heins, Cheng-Lun Na, Timothy E. Weaver, Marall Vedaie, Killian Hurley, Anne Hinds ...Show all 27 authors... , Scott J. Russo, Seunghyi Kook, William Zacharias, Matthias Ochs, Katrina Traber, Lee J. Quinton, Ana M. Crane, Brian R. Davis, Frances V. White, Jennifer Wambach, Jeffrey A. Whitsett, F. Sessions Cole, Edward E. Morrisey, Susan H. Guttentag, Michael F. Beers, Darrell N. Kotton; Show fewer authors
Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells.
Cell Stem Cell 21:472-488.e10(2017)

Cross-references
Encyclopedic resources Wikidata; Q93439149
Entry history
Entry creation24-May-2019
Last entry update14-Aug-2025
Version number14