Cellosaurus cell line C17 NKX2-1-GFP (CVCL

Cellosaurus C17 NKX2-1-GFP (CVCL_D3D6)

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Cell line name

C17 NKX2-1-GFP

Synonyms

C17 NG; C17 NKX2.1-GFP; iPS17 NKX2.1-GFP

Accession

CVCL_D3D6

Resource Identification Initiative

To cite this cell line use: C17 NKX2-1-GFP (RRID:CVCL_D3D6)

Comments

From: Center for Regenerative Medicine; Boston; USA.
Population: Caucasian.
Characteristics: Using TALEN a eGFP-pA-loxP-PGKp-PuroR-pA-loxP was inserted at the NKX2-1 translational start site.
Genetic integration: Method=TALEN; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Cell type: Fibroblast of skin; CL=CL_0002620.

Sequence variations

Mutation; HGNC; HGNC:1884; CFTR; Simple; p.Ile507del (c.1516_1518ATC[1]) (c.1519_1521delATC); ClinVar=VCV000007106; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:1884; CFTR; Simple; p.Phe508del (c.1521_1523delCTT); ClinVar=VCV000007105; Zygosity=Heterozygous (from parent cell line).

Disease

Cystic fibrosis (NCIt: C2975)
Cystic fibrosis (ORDO: Orphanet_586)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_WN85 (CF iPSC clone 17)
Children:

CVCL_WN83 (C17 NKX2-1-GFP SFTPC-tdTomato)

Sex of cell

Male

Age at sampling

17Y