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Cellosaurus MDCK.SUS2 (CVCL_WL71)

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Cell line name MDCK.SUS2
Accession CVCL_WL71
Resource Identification Initiative To cite this cell line use: MDCK.SUS2 (RRID:CVCL_WL71)
Comments Group: Serum/protein free medium cell line.
Group: Vaccine production cell line.
Characteristics: Adapted to growth in suspension in SMIF8 medium.
Doubling time: 24 hours (Note=In wave bioreactor), 37 hours (Note=In stirred-tank reactor) (PubMed=20638458); 24-26 hours (Note=In Smif8 medium) (PubMed=31047676).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_WL70 (MDCK.SUS1)
Children:
CVCL_WL72 (MDCK.Xeno)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=20638458; DOI=10.1016/j.vaccine.2010.07.004
Verena Lohr, Yvonne Genzel, Ilona Behrendt, Klaus Scharfenberg, Udo Reichl;
A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor.
Vaccine 28:6256-6264(2010)

PubMed=25994255; DOI=10.1007/s00253-015-6636-8
Rute Castro, Paulo Fernandes, Tanja Laske, Marcos F.Q. Sousa, Yvonne Genzel, Klaus Scharfenberg, Paula Marques Alves, Ana Sofia Coroadinha;
Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells.
Appl. Microbiol. Biotechnol. 99:7059-7068(2015)

PubMed=31047676; DOI=10.1016/j.vaccine.2019.04.054
Thomas Bissinger, Johannes Fritsch, Adrian Mihut, Yi-Xiao Wu, Xu-Ping Liu, Yvonne Genzel, Wen-Song Tan, Udo Reichl;
Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production.
Vaccine 37:7003-7010(2019)

Cross-references
Cell line databases/resources FCS-free; 41-14-330-1-16-12
Encyclopedic resources Wikidata; Q95987810
Entry history
Entry creation24-May-2019
Last entry update10-Apr-2025
Version number9