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Cellosaurus MDCK.SUS2 (CVCL_WL71)

[Text version]
Cell line name MDCK.SUS2
Accession CVCL_WL71
Resource Identification Initiative To cite this cell line use: MDCK.SUS2 (RRID:CVCL_WL71)
Comments Group: Serum/protein free medium cell line.
Group: Vaccine production cell line.
Characteristics: Adapted to growth in suspension in SMIF8 medium.
Doubling time: 24 hours (Note=In wave bioreactor), 37 hours (Note=In stirred-tank reactor) (PubMed=20638458).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel.
Hierarchy Parent: CVCL_WL70 (MDCK.SUS1)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line

PubMed=20638458; DOI=10.1016/j.vaccine.2010.07.004
Lohr V., Genzel Y., Behrendt I., Scharfenberg K., Reichl U.
A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor.
Vaccine 28:6256-6264(2010)

PubMed=25994255; DOI=10.1007/s00253-015-6636-8
Castro R., Fernandes P., Laske T., Sousa M.F.Q., Genzel Y., Scharfenberg K., Alves P.M., Coroadinha A.S.
Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells.
Appl. Microbiol. Biotechnol. 99:7059-7068(2015)

PubMed=31047676; DOI=10.1016/j.vaccine.2019.04.054
Bissinger T., Fritsch J., Mihut A., Wu Y.-X., Liu X.-P., Genzel Y., Tan W.-S., Reichl U.
Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production.
Vaccine 37:7003-7010(2019)

Cell line databases/resources FCS-free; 41-14-330-1-16-12
Encyclopedic resources Wikidata; Q95987810
Entry history
Entry creation24-May-2019
Last entry update05-Oct-2023
Version number7