DR   FCS-free; 41-14-330-1-16-12
DR   Wikidata; Q95987810
RX   PubMed=20638458;
RX   PubMed=25994255;
RX   PubMed=31047676;
CC   Group: Serum/protein free medium cell line.
CC   Group: Vaccine production cell line.
CC   Characteristics: Adapted to growth in suspension in SMIF8 medium.
CC   Doubling time: 24 hours (Note=In wave bioreactor), 37 hours (Note=In stirred-tank reactor) (PubMed=20638458).
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
CC   Breed/subspecies: Cocker Spaniel.
OX   NCBI_TaxID=9615; ! Canis lupus familiaris (Dog)
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 24-05-19; Last updated: 05-10-23; Version: 7
RX   PubMed=20638458; DOI=10.1016/j.vaccine.2010.07.004;
RA   Lohr V., Genzel Y., Behrendt I., Scharfenberg K., Reichl U.;
RT   "A new MDCK suspension line cultivated in a fully defined medium in
RT   stirred-tank and wave bioreactor.";
RL   Vaccine 28:6256-6264(2010).
RX   PubMed=25994255; DOI=10.1007/s00253-015-6636-8;
RA   Castro R., Fernandes P., Laske T., Sousa M.F.Q., Genzel Y.,
RA   Scharfenberg K., Alves P.M., Coroadinha A.S.;
RT   "Production of canine adenovirus type 2 in serum-free suspension
RT   cultures of MDCK cells.";
RL   Appl. Microbiol. Biotechnol. 99:7059-7068(2015).
RX   PubMed=31047676; DOI=10.1016/j.vaccine.2019.04.054;
RA   Bissinger T., Fritsch J., Mihut A., Wu Y.-X., Liu X.-P., Genzel Y.,
RA   Tan W.-S., Reichl U.;
RT   "Semi-perfusion cultures of suspension MDCK cells enable high cell
RT   concentrations and efficient influenza A virus production.";
RL   Vaccine 37:7003-7010(2019).