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Cellosaurus CWR22Rv1-AR-EK (CVCL_WK44)

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Cell line name CWR22Rv1-AR-EK
Synonyms CWR22Rv1-Androgen Receptor-Exon Knockout
Accession CVCL_WK44
Resource Identification Initiative To cite this cell line use: CWR22Rv1-AR-EK (RRID:CVCL_WK44)
Comments Population: Caucasian.
Characteristics: Has lost the ability to express full-length AR (FL-AR), but retains that of expressing alternatively spliced forms of the AR termed AR variants (AR-Vs).
Characteristics: Using CRISPR/Cas9 a stop codon was introduced downstream of the DNA-binding domain in exon 5 of the AR gene.
Derived from site: In situ; Prostate; UBERON=UBERON_0002367.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Leu597Arg (c.1790T>G); ClinVar=VCV000013968; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7133; KMT2D; Simple; p.Arg1252Ter (c.3754C>T); ClinVar=VCV000167228; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7133; KMT2D; Simple; p.Gln3158Ter (c.9472C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Gln546Arg (c.1637A>G); ClinVar=VCV000045466; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln331Arg (c.992A>G); ClinVar=VCV000421319; Zygosity=Heterozygous (from parent cell line).
Disease Prostate carcinoma (NCIt: C4863)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1045 (22Rv1)
Sex of cell Male
Age at sampling Adult
Category Cancer cell line
STR profile Source(s): PubMed=31006810

Markers:
AmelogeninX,Y
CSF1PO10,11
D5S81811,12
D7S82010,11
D13S3179,12
D16S53912
D21S1130
TH016,9.3
TPOX8
vWA15,21

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Publications

PubMed=31006810; DOI=10.1093/nar/gkz286; PMCID=PMC6582326
Evangelia Kounatidou, Sirintra Nakjang, Stuart R.C. McCracken, Scott M. Dehm, Craig N. Robson, Dominic Jones, Luke Gaughan;
A novel CRISPR-engineered prostate cancer cell line defines the AR-V transcriptome and identifies PARP inhibitor sensitivities.
Nucleic Acids Res. 47:5634-5647(2019)

Cross-references
Cell line databases/resources cancercelllines; CVCL_WK44
Encyclopedic resources Wikidata; Q93464997
Entry history
Entry creation24-May-2019
Last entry update19-Dec-2024
Version number10