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Cellosaurus CRISPRi Gen3C A3 (CVCL_VM42)

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Cell line name CRISPRi Gen3C A3
Synonyms WTC CRISPRi Gen3 clone A3; 3C-A3
Accession CVCL_VM42
Resource Identification Initiative To cite this cell line use: CRISPRi Gen3C A3 (RRID:CVCL_VM42)
Comments Population: Japanese.
Characteristics: Cell line with constitutive CRISPR interference (CRISPRi).
Characteristics: Using TALEN a construct containing a CAG promoter and dCas9-KRAB is introduced in the AAVS1 safe harbor locus.
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697
Mohammad A. Mandegar, Nathaniel Huebsch, Ekaterina B. Frolov, Edward Shin, Annie Truong, Michael P. Olvera, Amanda H. Chan, Yuichiro Miyaoka, Kristin Holmes, C. Ian Spencer, Luke M. Judge ...Show all 22 authors... , David Ezra Gordon, Tilde Veng Eskildsen, Jacqueline E. Villalta, Max A. Horlbeck, Luke A. Gilbert, Nevan J. Krogan, Soren P. Sheikh, Jonathan S. Weissman, Lei S. Qi, Po-Lin So, Bruce R. Conklin; Show fewer authors
CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs.
Cell Stem Cell 18:541-553(2016)

Cross-references
Encyclopedic resources Wikidata; Q93458772
Entry history
Entry creation07-Sep-2018
Last entry update10-Sep-2024
Version number9