ID   CRISPRi Gen3C A3
AC   CVCL_VM42
SY   WTC CRISPRi Gen3 clone A3; 3C-A3
DR   Wikidata; Q93458772
RX   PubMed=26971820;
CC   Population: Japanese.
CC   Characteristics: Cell line with constitutive CRISPR interference (CRISPRi).
CC   Characteristics: Using TALEN a construct containing a CAG promoter and dCas9-KRAB is introduced in the AAVS1 locus.
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_Y803 ! GM25256
SX   Male
AG   30Y
CA   Induced pluripotent stem cell
DT   Created: 07-09-18; Last updated: 30-01-24; Version: 7
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RX   PubMed=26971820; DOI=10.1016/j.stem.2016.01.022;
RA   Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A.,
RA   Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I.,
RA   Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A.,
RA   Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S.,
RA   So P.-L., Conklin B.R.;
RT   "CRISPR interference efficiently induces specific and reversible gene
RT   silencing in human iPSCs.";
RL   Cell Stem Cell 18:541-553(2016).
//