Home  |  Contact

Cellosaurus CRISPRi Gen2C 1 (CVCL_VM40)

[Text version]

Cell line name CRISPRi Gen2C 1
Synonyms WTC CRISPRi Gen2 clone 1; 2C-1
Accession CVCL_VM40
Resource Identification Initiative To cite this cell line use: CRISPRi Gen2C 1 (RRID:CVCL_VM40)
Comments Population: Japanese.
Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB is introduced in the AAVS1 locus.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from sampling site: Leg; skin. Cell type=Fibroblast.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell

PubMed=26971820; DOI=10.1016/j.stem.2016.01.022
Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A., Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I., Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A., Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S., So P.-L., Conklin B.R.
CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs.
Cell Stem Cell 18:541-553(2016)

Encyclopedic resources Wikidata; Q93458768
Entry history
Entry creation07-Sep-2018
Last entry update17-Mar-2022
Version number4