ID   CRISPRi Gen2C 1
AC   CVCL_VM40
SY   WTC CRISPRi Gen2 clone 1; 2C-1
DR   Wikidata; Q93458768
RX   PubMed=26971820;
CC   Population: Japanese.
CC   Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
CC   Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB is introduced in the AAVS1 locus.
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_Y803 ! GM25256
SX   Male
AG   30Y
CA   Induced pluripotent stem cell
DT   Created: 07-09-18; Last updated: 30-01-24; Version: 7
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RX   PubMed=26971820; DOI=10.1016/j.stem.2016.01.022;
RA   Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A.,
RA   Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I.,
RA   Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A.,
RA   Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S.,
RA   So P.-L., Conklin B.R.;
RT   "CRISPR interference efficiently induces specific and reversible gene
RT   silencing in human iPSCs.";
RL   Cell Stem Cell 18:541-553(2016).
//