Cellosaurus cell line CRISPRi Gen1B 3 (CVCL

Cellosaurus CRISPRi Gen1B 3 (CVCL_VM36)

Cross-references
Cell line name	CRISPRi Gen1B 3
Synonyms	WTB CRISPRi Gen1 clone 3; 1B-3
Accession	CVCL_VM36
Resource Identification Initiative	To cite this cell line use: CRISPRi Gen1B 3 (RRID:CVCL_VM36)
Comments	Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi). Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus. Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_VM30 (WTB6)
Sex of cell	Female
Age at sampling	Age unspecified
Category	Induced pluripotent stem cell
Publications	PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697 Mohammad A. Mandegar, Nathaniel Huebsch, Ekaterina B. Frolov, Edward Shin, Annie Truong, Michael P. Olvera, Amanda H. Chan, Yuichiro Miyaoka, Kristin Holmes, C. Ian Spencer, Luke M. Judge ...Show all 22 authors... , David Ezra Gordon, Tilde Veng Eskildsen, Jacqueline E. Villalta, Max A. Horlbeck, Luke A. Gilbert, Nevan J. Krogan, Soren P. Sheikh, Jonathan S. Weissman, Lei S. Qi, Po-Lin So, Bruce R. Conklin; Show fewer authors CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs. Cell Stem Cell 18:541-553(2016)
Encyclopedic resources	Wikidata; Q93458761
Entry history
Entry creation	07-Sep-2018
Last entry update	14-Aug-2025
Version number	9