ID   CRISPRi Gen1B 3
AC   CVCL_VM36
SY   WTB CRISPRi Gen1 clone 3; 1B-3
DR   Wikidata; Q93458761
RX   PubMed=26971820;
CC   Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
CC   Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus.
CC   Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_VM30 ! WTB6
SX   Female
AG   Age unspecified
CA   Induced pluripotent stem cell
DT   Created: 07-09-18; Last updated: 10-04-25; Version: 8
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RX   PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697;
RA   Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A.,
RA   Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I.,
RA   Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A.,
RA   Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S.,
RA   So P.-L., Conklin B.R.;
RT   "CRISPR interference efficiently induces specific and reversible gene
RT   silencing in human iPSCs.";
RL   Cell Stem Cell 18:541-553(2016).
//