Cellosaurus cell line CRISPRn Gen1C 22 (CVCL

Cellosaurus CRISPRn Gen1C 22 (CVCL_VM34)

Cross-references
Cell line name	CRISPRn Gen1C 22
Synonyms	WTC CRISPRn Gen1 clone 22; CRISPRi (Gen1C 22); 1C-n22
Accession	CVCL_VM34
Resource Identification Initiative	To cite this cell line use: CRISPRn Gen1C 22 (RRID:CVCL_VM34)
Comments	Population: Japanese. Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9 is introduced in the AAVS1 safe harbor locus. Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9). Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511. Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_Y803 (GM25256)
Sex of cell	Male
Age at sampling	30Y
Category	Induced pluripotent stem cell
Publications	PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697 Mohammad A. Mandegar, Nathaniel Huebsch, Ekaterina B. Frolov, Edward Shin, Annie Truong, Michael P. Olvera, Amanda H. Chan, Yuichiro Miyaoka, Kristin Holmes, C. Ian Spencer, Luke M. Judge ...Show all 22 authors... , David Ezra Gordon, Tilde Veng Eskildsen, Jacqueline E. Villalta, Max A. Horlbeck, Luke A. Gilbert, Nevan J. Krogan, Soren P. Sheikh, Jonathan S. Weissman, Lei S. Qi, Po-Lin So, Bruce R. Conklin; Show fewer authors CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs. Cell Stem Cell 18:541-553(2016)
Encyclopedic resources	Wikidata; Q93458783
Entry history
Entry creation	07-Sep-2018
Last entry update	14-Aug-2025
Version number	11