ID   CRISPRn Gen1C 22
AC   CVCL_VM34
SY   WTC CRISPRn Gen1 clone 22; CRISPRi (Gen1C 22); 1C-n22
DR   Wikidata; Q93458783
RX   PubMed=26971820;
CC   Population: Japanese.
CC   Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9 is introduced in the AAVS1 safe harbor locus.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
CC   Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_Y803 ! GM25256
SX   Male
AG   30Y
CA   Induced pluripotent stem cell
DT   Created: 07-09-18; Last updated: 10-04-25; Version: 10
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RX   PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697;
RA   Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A.,
RA   Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I.,
RA   Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A.,
RA   Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S.,
RA   So P.-L., Conklin B.R.;
RT   "CRISPR interference efficiently induces specific and reversible gene
RT   silencing in human iPSCs.";
RL   Cell Stem Cell 18:541-553(2016).
//