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Cellosaurus MYCN-2 Tet-on (CVCL_VL18)

[Text version]
Cell line name MYCN-2 Tet-on
Synonyms MYCN-2; MYCN2
Accession CVCL_VL18
Resource Identification Initiative To cite this cell line use: MYCN-2 Tet-on (RRID:CVCL_VL18)
Comments Population: Caucasian.
Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7559; MYCN.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Mutation; HGNC; HGNC:427; ALK; Simple; p.Phe1174Leu (c.3522C>A); ClinVar=VCV000217852; Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Neuroblastoma (NCIt: C3270)
Neuroblastoma (ORDO: Orphanet_635)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0524 (SH-EP)
Sex of cell Female
Age at sampling 4Y
Category Cancer cell line
Publications

PubMed=15644444; DOI=10.1073/pnas.0405495102; PMCID=PMC545522
Andrew Slack, Zao-Wen Chen, Roberto Tonelli, Martin Pule, Lisa Hunt, Andrea Pession, Jason M. Shohet;
The p53 regulatory gene MDM2 is a direct transcriptional target of MYCN in neuroblastoma.
Proc. Natl. Acad. Sci. U.S.A. 102:731-736(2005)

PubMed=23440295; DOI=10.3892/ijo.2013.1835; PMCID=PMC3622674
Dana-Lynn T. Koomoa, Dirk Geerts, Ingo Lange, Jan Koster, Anthony E. Pegg, David J. Feith, Andre S. Bachmann;
DFMO/eflornithine inhibits migration and invasion downstream of MYCN and involves p27Kip1 activity in neuroblastoma.
Int. J. Oncol. 42:1219-1228(2013)

Cross-references
Cell line databases/resources cancercelllines; CVCL_VL18
Anatomy/cell type resources BTO; BTO_0006118
Encyclopedic resources Wikidata; Q95991616
Entry history
Entry creation07-Sep-2018
Last entry update14-Aug-2025
Version number11