Cellosaurus MEF (DR4) IRR (CVCL_VK72)
| Cell line name | MEF (DR4) IRR |
|---|---|
| Synonyms | DR4 IRR; DR-4 IRR |
| Accession | CVCL_VK72 |
| Resource Identification Initiative | To cite this cell line use: MEF (DR4) IRR (RRID:CVCL_VK72) |
| Comments | Characteristics: Used as feeder cells to support the growth of stem cells in the undifferentiated state. Characteristics: Growth-arrested by irradiation with 12000 rads. Characteristics: Expresses transgenes that make this cell line resistant to three different drugs: geneticin (G418), puromycin and hygromycin. KO of Hprt1 makes the cell line resistant to 6-tioguanine (from parent cell line). Knockout cell: Method=KO mouse; MGI; MGI:96217; Hprt1. Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: ChEBI; CHEBI_27504; Mitomycin C (MMC; MitC). Derived from site: In situ; Embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: DR4. |
| Hierarchy | Parent: CVCL_5277 (MEF (DR4)) |
| Sex of cell | Mixed sex |
| Age at sampling | 14FD |
| Category | Finite cell line |
| Cross-references | |
| Cell line collections (Providers) | ATCC; SCRC-1045.1 - Discontinued |
| Cell line databases/resources | CCRID; 3101MOUSCSP108R |
| Encyclopedic resources | Wikidata; Q95988434 |
| Entry history | |
| Entry creation | 07-Sep-2018 |
| Last entry update | 10-Apr-2025 |
| Version number | 12 |