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Cellosaurus FM3A tsFT20 (CVCL_VI63)

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Cell line name FM3A tsFT20
Synonyms tsFT20
Accession CVCL_VI63
Resource Identification Initiative To cite this cell line use: FM3A tsFT20 (RRID:CVCL_VI63)
Comments Characteristics: Temperature sensitive phenotype. Stop growing at 39 Celsius. Defective in DNA replication at the non-permissive temperature (PubMed=3856858).
Doubling time: 16 hours (PubMed=3856858).
Transformant: ChEBI; CHEBI_21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Sequence variations
  • Mutation; MGI; MGI:99660; Pola1; Simple; p.Ser1180Phe (c.3539C>T); Zygosity=Unspecified (PubMed=8125989).
Disease Malignant neoplasms of the mouse mammary gland (NCIt: C21678)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H/He.
Hierarchy Parent: CVCL_3869 (FM3A)
Sex of cell Female
Age at sampling Age unspecified
Category Cancer cell line
Publications

PubMed=3856858; DOI=10.1073/pnas.82.6.1761; PMCID=PMC397352
Yasufumi Murakami, Hideyo Yasuda, Hiroshi Miyazawa, Fumio Hanaoka, Masa-atsu Yamada;
Characterization of a temperature-sensitive mutant of mouse FM3A cells defective in DNA replication.
Proc. Natl. Acad. Sci. U.S.A. 82:1761-1765(1985)

PubMed=8125989; DOI=10.1016/S0021-9258(17)37335-0
Masako Izumi, Hiroshi Miyazawa, Shoko Harakawa, Fumio Yatagai, Fumio Hanaoka;
Identification of a point mutation in the cDNA of the catalytic subunit of DNA polymerase alpha from a temperature-sensitive mouse FM3A cell line.
J. Biol. Chem. 269:7639-7644(1994)

Cross-references
Encyclopedic resources Wikidata; Q93555121
Entry history
Entry creation07-Sep-2018
Last entry update14-Aug-2025
Version number8