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Cellosaurus HMGEC (CVCL_VF64)

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Cell line name HMGEC
Synonyms IHMGEC; Human Meibomian Gland Epithelial Cells
Accession CVCL_VF64
Resource Identification Initiative To cite this cell line use: HMGEC (RRID:CVCL_VF64)
Comments Doubling time: 27.39 hours (PubMed=20335607); ~3-7 days (ATCC=CRL-3472).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Meibomian gland; UBERON=UBERON_0001818.
Cell type: Epithelial cell; CL=CL_0000066.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 58Y
Category Transformed cell line
STR profile Source(s): PubMed=29787296

Markers:
AmelogeninX,Y
CSF1PO10,12
D3S135815,17
D5S81810,13
D7S8208,11
D8S117913,15
D13S3179,14
D16S5398,11
D18S5112,16
D21S1129,30.2
FGA23,24
Penta D8,12
Penta E5,14
TH016,9.3
TPOX8,9
vWA16,17

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Publications

PubMed=20335607; DOI=10.1167/iovs.09-5108; PMCID=PMC2910637
Shao-Hui Liu, Mark P. Hatton, Payal Khandelwal, David A. Sullivan;
Culture, immortalization, and characterization of human meibomian gland epithelial cells.
Invest. Ophthalmol. Vis. Sci. 51:3993-4005(2010)

PubMed=26042605; DOI=10.1371/journal.pone.0128096; PMCID=PMC4456149
Ulrike Hampel, Antje Schroder, Todd Mitchell, Simon Brown, Peta Snikeris, Fabian Garreis, Carolina Kunnen, Mark Willcox, Friedrich Paulsen;
Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line.
PLoS ONE 10:e0128096.1-e0128096.18(2015)

PubMed=28363775; DOI=10.1016/j.exer.2017.03.011
Ulrike Hampel, Fabian Garreis;
The human meibomian gland epithelial cell line as a model to study meibomian gland dysfunction.
Exp. Eye Res. 163:46-52(2017)

PubMed=29625457; DOI=10.1167/iovs.17-23266
Nagayoshi Asano, Ulrike Hampel, Fabian Garreis, Antje Schroder, Martin Schicht, Christian M. Hammer, Friedrich Paulsen;
Differentiation patterns of immortalized human meibomian gland epithelial cells in three-dimensional culture.
Invest. Ophthalmol. Vis. Sci. 59:1343-1353(2018)

PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053
Alison M. McDermott, Hasna Baidouri, Ashley M. Woodward, Wendy R. Kam, Yang Liu, Xiao-Min Chen, Jillian F. Ziemanski, Kerry Vistisen, Linda D. Hazlett, Kelly K. Nichols, Pablo Argueso, David A. Sullivan;
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

Cross-references
Cell line collections (Providers) ATCC; CRL-3472
Encyclopedic resources Wikidata; Q54889933
Entry history
Entry creation14-May-2018
Last entry update14-Aug-2025
Version number13