Cellosaurus MEF GFP-LC3-RFP-LC3deltaG (CVCL_UP52)
| Cell line name | MEF GFP-LC3-RFP-LC3deltaG |
|---|---|
| Synonyms | GFP-LC3-RFP-LC3deltaG MEF |
| Accession | CVCL_UP52 |
| Resource Identification Initiative | To cite this cell line use: MEF GFP-LC3-RFP-LC3deltaG (RRID:CVCL_UP52) |
| Comments | Characteristics: Stably expresses EGFP-LC3-mRFP-LC3delta, an autophagic flux probe. GFP-LC3 is fused to the N-terminus of RFP-LC3 whose C-terminal glycine is deleted. When expressed, the probe it is separated into EGFP-LC3 and mRFP-LC3deltaG by the action of endogenous ATG4 family proteases that cleave the peptide bond after the C-terminal glycine of LC3 in the cytosol producing equimolar amounts of EGFP-LC3 and mRFP-LC3deltaG (PubMed=27818143). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Genetic integration: Method=Transduction; Gene=RGD; 735183; Map1lc3a. Derived from site: In situ; Embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6. |
| Sex of cell | Sex unspecified |
| Age at sampling | 13-14FD |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=27818143; DOI=10.1016/j.molcel.2016.09.037 |
| Cross-references | |
| Cell line collections (Providers) | RCB; RCB4696 |
| Encyclopedic resources | Wikidata; Q95988436 |
| Entry history | |
| Entry creation | 25-Feb-2019 |
| Last entry update | 10-Apr-2025 |
| Version number | 11 |