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Cellosaurus MEF GFP-LC3-RFP-LC3deltaG (CVCL_UP52)

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Cell line name MEF GFP-LC3-RFP-LC3deltaG
Synonyms GFP-LC3-RFP-LC3deltaG MEF
Accession CVCL_UP52
Resource Identification Initiative To cite this cell line use: MEF GFP-LC3-RFP-LC3deltaG (RRID:CVCL_UP52)
Comments Characteristics: Stably expresses EGFP-LC3-mRFP-LC3delta, an autophagic flux probe. GFP-LC3 is fused to the N-terminus of RFP-LC3 whose C-terminal glycine is deleted. When expressed, the probe it is separated into EGFP-LC3 and mRFP-LC3deltaG by the action of endogenous ATG4 family proteases that cleave the peptide bond after the C-terminal glycine of LC3 in the cytosol producing equimolar amounts of EGFP-LC3 and mRFP-LC3deltaG.
Transfected with: RGD; 735183; Map1lc3a.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; A0A4V4ND72; mRFP1.
Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Sex of cell Sex unspecified
Age at sampling 13-14FD
Category Spontaneously immortalized cell line

PubMed=27818143; DOI=10.1016/j.molcel.2016.09.037
Kaizuka T., Morishita H., Hama Y., Tsukamoto S., Matsui T., Toyota Y., Kodama A., Ishihara T., Mizushima T., Mizushima N.
An autophagic flux probe that releases an internal control.
Mol. Cell 64:835-849(2016)

Cell line collections (Providers) RCB; RCB4696
Encyclopedic resources Wikidata; Q95988436
Entry history
Entry creation25-Feb-2019
Last entry update29-Jun-2023
Version number7