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Cellosaurus 82-6pBlox (CVCL_U637)

[Text version]
Cell line name 82-6pBlox
Synonyms 82-6pBloxTSH; 82-6 hTERT
Accession CVCL_U637
Resource Identification Initiative To cite this cell line use: 82-6pBlox (RRID:CVCL_U637)
Comments Characteristics: Transduced with a lentiviral vector containing a LTR-loxP-hTERT-SV40p-hisD-Pac-LTR construct. When transfected with Cre recombinase the cells loose telomerase activity and histidinol sensitivity due to the excision of the hTERT and hisD genes. Simultaneously, the cells acquire puromycin resistance because the excision brings the PuroR gene under the control of the retroviral promoter (LTR).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06988; Escherichia coli hisD.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_U636 (82-6)
Sex of cell Male
Age at sampling <1M
Category Telomerase immortalized cell line
Publications

PubMed=12034742; DOI=10.1074/jbc.M203747200
Rubio M.A., Kim S.-H., Campisi J.
Reversible manipulation of telomerase expression and telomere length Implications for the ionizing radiation response and replicative senescence of human cells.
J. Biol. Chem. 277:28609-28617(2002)

PubMed=17870066; DOI=10.1016/j.yexcr.2007.08.004; PMCID=PMC2228272
Huang S., Risques R.A., Martin G.M., Rabinovitch P.S., Oshima J.
Accelerated telomere shortening and replicative senescence in human fibroblasts overexpressing mutant and wild-type lamin A.
Exp. Cell Res. 314:82-91(2008)

PubMed=23847654; DOI=10.3389/fgene.2013.00129; PMCID=PMC3701863
Saha B., Zitnik G., Johnson S., Nguyen Q., Risques R.A., Martin G.M., Oshima J.
DNA damage accumulation and TRF2 degradation in atypical Werner syndrome fibroblasts with LMNA mutations.
Front. Genet. 4:129.1-129.12(2013)

Cross-references
Encyclopedic resources Wikidata; Q54605317
Entry history
Entry creation16-Apr-2014
Last entry update19-Dec-2024
Version number10