ID   82-6pBlox
AC   CVCL_U637
SY   82-6pBloxTSH; 82-6 hTERT
DR   Wikidata; Q54605317
RX   PubMed=12034742;
RX   PubMed=17870066;
RX   PubMed=23847654;
CC   Characteristics: Transduced with a lentiviral vector containing a LTR-loxP-hTERT-SV40p-hisD-Pac-LTR construct. When transfected with Cre recombinase the cells loose telomerase activity and histidinol sensitivity due to the excision of the hTERT and hisD genes. Simultaneously, the cells acquire puromycin resistance because the excision brings the PuroR gene under the control of the retroviral promoter (LTR).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06988; Escherichia coli hisD.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
CC   Cell type: Fibroblast of foreskin; CL=CL_1001608.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_U636 ! 82-6
SX   Male
AG   <1M
CA   Telomerase immortalized cell line
DT   Created: 16-04-14; Last updated: 19-12-24; Version: 10
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RX   PubMed=12034742; DOI=10.1074/jbc.M203747200;
RA   Rubio M.A., Kim S.-H., Campisi J.;
RT   "Reversible manipulation of telomerase expression and telomere length.
RT   Implications for the ionizing radiation response and replicative
RT   senescence of human cells.";
RL   J. Biol. Chem. 277:28609-28617(2002).
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RX   PubMed=17870066; DOI=10.1016/j.yexcr.2007.08.004; PMCID=PMC2228272;
RA   Huang S., Risques R.A., Martin G.M., Rabinovitch P.S., Oshima J.;
RT   "Accelerated telomere shortening and replicative senescence in human
RT   fibroblasts overexpressing mutant and wild-type lamin A.";
RL   Exp. Cell Res. 314:82-91(2008).
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RX   PubMed=23847654; DOI=10.3389/fgene.2013.00129; PMCID=PMC3701863;
RA   Saha B., Zitnik G., Johnson S., Nguyen Q., Risques R.A., Martin G.M.,
RA   Oshima J.;
RT   "DNA damage accumulation and TRF2 degradation in atypical Werner
RT   syndrome fibroblasts with LMNA mutations.";
RL   Front. Genet. 4:129.1-129.12(2013).
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