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Cellosaurus NCC16 (CVCL_U219)

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Cell line name NCC16
Accession CVCL_U219
Resource Identification Initiative To cite this cell line use: NCC16 (RRID:CVCL_U219)
Comments Problematic cell line: Contaminated. Shown to be a PHK16-0b derivative (PubMed=20143388).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00331.
Population: Caucasian.
Characteristics: Transfected with the plasmid pMHPV16d, which contains two copies of the complete HPV16 genome in a head-to-tail configuration (from parent cell line).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Caution: Replaced by NCC16-P11.
Derived from site: In situ; Skin, epidermis; UBERON=UBERON_0001003.
Cell type: Keratinocyte; CL=CL_0000312.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_3123 (PHK16-0b)
Sex of cell Male
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): JCRB=JCRB0142

Markers:
AmelogeninX,Y
CSF1PO12,13
D5S8189,11
D7S8209,11
D13S31711
D16S53911,12
TH0110
TPOX8
vWA16

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=9310137; DOI=10.1111/j.1349-7006.1997.tb00432.x; PMCID=PMC5921480
Yujiro Ohta, Kouichiro Tsutsumi, Keiji Kikuchi, Shigeru Yasumoto;
Two distinct human uterine cervical epithelial cell lines established after transfection with human papillomavirus 16 DNA.
Jpn. J. Cancer Res. 88:644-651(1997)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections (Providers) JCRB; JCRB0142 - Discontinued
JCRB; NIHS0204 - Discontinued
Biological sample resources BioSample; SAMN03151898
BioSample; SAMN03470809
Encyclopedic resources Wikidata; Q54907639
Entry history
Entry creation03-Feb-2014
Last entry update14-Aug-2025
Version number18