Cellosaurus NMuMG/Fucci2 (CVCL_U135)
| Cell line name | NMuMG/Fucci2 |
|---|---|
| Synonyms | NMuMG/Fucci2 #5; NMuMG-Fucci2 |
| Accession | CVCL_U135 |
| Resource Identification Initiative | To cite this cell line use: NMuMG/Fucci2 (RRID:CVCL_U135) |
| Comments | Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci2 (Fluorescent Ubiquitination-based Cell Cycle Indicator 2) construct. Characteristics: Transfected with the Fucci2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (AA 30-120) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (AA 1-110). Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transfection; Gene=FPbase; YUJWJ; Venus (Note=Bright yellow fluorescent protein derived from GFP). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del). Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: Namru. |
| Hierarchy | Parent: CVCL_0075 (NMuMG) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=21226962; DOI=10.1186/1471-2121-12-2; PMCID=PMC3277280 PubMed=24786503; DOI=10.1101/pdb.prot080408 |
| Cross-references | |
| Cell line collections (Providers) | RCB; RCB2868 |
| Cell line databases/resources | CLO; CLO_0050968 |
| Encyclopedic resources | Wikidata; Q54930851 |
| Entry history | |
| Entry creation | 03-Feb-2014 |
| Last entry update | 10-Apr-2025 |
| Version number | 13 |