ID   NMuMG/Fucci2
AC   CVCL_U135
SY   NMuMG/Fucci2 #5; NMuMG-Fucci2
DR   CLO; CLO_0050968
DR   RCB; RCB2868
DR   Wikidata; Q54930851
RX   PubMed=21226962;
RX   PubMed=24786503;
CC   Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci2 (Fluorescent Ubiquitination-based Cell Cycle Indicator 2) construct.
CC   Characteristics: Transfected with the Fucci2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (AA 30-120) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (AA 1-110).
CC   Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transfection; Gene=FPbase; YUJWJ; Venus (Note=Bright yellow fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
CC   Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
CC   Breed/subspecies: Namru.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0075 ! NMuMG
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 03-02-14; Last updated: 10-04-25; Version: 13
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RX   PubMed=21226962; DOI=10.1186/1471-2121-12-2; PMCID=PMC3277280;
RA   Sakaue-Sawano A., Kobayashi T., Ohtawa K., Miyawaki A.;
RT   "Drug-induced cell cycle modulation leading to cell-cycle arrest,
RT   nuclear mis-segregation, or endoreplication.";
RL   BMC Cell Biol. 12:2.1-2.12(2011).
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RX   PubMed=24786503; DOI=10.1101/pdb.prot080408;
RA   Sakaue-Sawano A., Miyawaki A.;
RT   "Visualizing spatiotemporal dynamics of multicellular cell-cycle
RT   progressions with Fucci technology.";
RL   Cold Spring Harb. Protoc. 2014:Pdb.prot080408-Pdb.prot080408(2014).
//