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Cellosaurus A3R5.7 (CVCL_S540)

[Text version]
Cell line name A3R5.7
Synonyms A3.01/R5.7; A3R5
Accession CVCL_S540
Resource Identification Initiative To cite this cell line use: A3R5.7 (RRID:CVCL_S540)
Comments Population: Caucasian.
Characteristics: Expresses CCR5 under the control of a CMV promoter (PubMed=24312168).
Characteristics: Useful for the detection of weak neutralizing antibody responses against tier 2 strains of HIV-1 (PubMed=24607608).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1606; CCR5.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene fusion; HGNC; HGNC:13222; BCL11B + HGNC; HGNC:2488; NKX2-5; Name(s)=NKX2.5-BCL11B (from parent cell line).
  • Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg465Cys (c.1393C>T); ClinVar=VCV000376414; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:3765; FLT3; Simple; p.Ala627Thr (c.1879G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Leu1593_Arg1594insProArgLeuProHisAsnSerSerPheHisPheLeu (c.4780_4781ins36); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Pro2412Thr (c.7234C>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_6244 (CEM A3.01)
Sex of cell Female
Age at sampling 3Y11M
Category Cancer cell line
Publications

PubMed=24312168; DOI=10.1371/journal.pone.0077756; PMCID=PMC3842913
McLinden R.J., LaBranche C.C., Chenine A.-L., Polonis V.R., Eller M.A., Wieczorek L., Ochsenbauer-Jambor C., Kappes J.C., Perfetto S., Montefiori D.C., Michael N.L., Kim J.H.
Detection of HIV-1 neutralizing antibodies in a human CD4(+)/CXCR4(+)/CCR5(+) T-lymphoblastoid cell assay system.
PLoS ONE 8:e77756.1-e77756.13(2013)

PubMed=24607608; DOI=10.1016/j.jim.2014.02.013; PMCID=PMC4138262
Sarzotti-Kelsoe M., Daniell X.-J., Todd C.A., Bilska M., Martelli A., LaBranche C.C., Perez L.G., Ochsenbauer-Jambor C., Kappes J.C., Rountree W., Denny T.N., Montefiori D.C.
Optimization and validation of a neutralizing antibody assay for HIV-1 in A3R5 cells.
J. Immunol. Methods 409:147-160(2014)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-12386
Cell line databases/resources cancercelllines; CVCL_S540
Encyclopedic resources Wikidata; Q54606881
Entry history
Entry creation03-Feb-2014
Last entry update19-Dec-2024
Version number14