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Cellosaurus HEK293-YFV-prM/E-opt (CVCL_RZ86)

[Text version]
Cell line name HEK293-YFV-prM/E-opt
Accession CVCL_RZ86
Resource Identification Initiative To cite this cell line use: HEK293-YFV-prM/E-opt (RRID:CVCL_RZ86)
Comments Characteristics: Expresses the YFV prM/E proteins and capable of trans-packaging the transcribed RNA of a YFV reporter replicon, thereby generating pseudo-infectious viral particles.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03314; Yellow fever virus (strain 17D vaccine) genome polyprotein (Note=AA 102-778; protein prM + protein E, codons optimized for expression).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=29513845; DOI=10.1590/0037-8682-0220-2017
Sabrina Ribeiro de Almeida Queiroz, Jose Valter Joaquim Silva Jr., Andrea Nazare Monteiro Rangel da Silva, Amanda Gomes de Oliveira Carvalho, Jefferson Jose da Silva Santos, Laura Helena Vega Gonzales Gil;
Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation.
Rev. Soc. Bras. Med. Trop. 51:66-70(2018)

Cross-references
Encyclopedic resources Wikidata; Q54882461
Entry history
Entry creation14-May-2018
Last entry update14-Aug-2025
Version number9