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Cellosaurus A549-Mx2/EGFP L2G9 (CVCL_RX09)

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Cell line name A549-Mx2/EGFP L2G9
Synonyms A549L2G9; A549 L2G9; L2G9
Accession CVCL_RX09
Resource Identification Initiative To cite this cell line use: A549-Mx2/EGFP L2G9 (RRID:CVCL_RX09)
Comments Population: Caucasian.
Biotechnology: Used for a reporter gene assay (RGA) of the human type 1 interferon potency (PubMed=27346232).
Characteristics: Expression of eGFP is under the control of the control of the mouse Mx2 promoter (PubMed=27346232).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Publications

PubMed=22373148; DOI=10.1186/1753-6561-5-S8-P4; PMCID=PMC3284889
Maria Burgi, Claudio Prieto, Marcos Oggero, Mariela Bollati-Fogolin, Marina Etcheverrigaray, Ricardo Kratje;
New reporter cell clones to determine the biological activity of human type I interferons.
BMC Proc. 5 Suppl. 8:P4-P4(2011)

PubMed=27346232; DOI=10.1016/j.jbiotec.2016.06.021
Maria Burgi, Viktor A. Zapol'skii, Bettina Hinkelmann, Mario Koster, Dieter E. Kaufmann, Florenz Sasse, Hansjorg Hauser, Marina Etcheverrigaray, Ricardo Kratje, Mariela Bollati-Fogolin, Marcos Oggero;
Screening and characterization of molecules that modulate the biological activity of IFNs-I.
J. Biotechnol. 233:6-16(2016)

Cross-references
Cell line databases/resources cancercelllines; CVCL_RX09
Encyclopedic resources Wikidata; Q54607001
Entry history
Entry creation05-Mar-2018
Last entry update14-Aug-2025
Version number13