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Cellosaurus 293T R-Spondin1 expressing (CVCL_RU09)

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Cell line name 293T R-Spondin1 expressing
Synonyms R-Spondin1 expressing 293T
Accession CVCL_RU09
Resource Identification Initiative To cite this cell line use: 293T R-Spondin1 expressing (RRID:CVCL_RU09)
Comments Characteristics: Expresses mouse Fc-Rspo1 which consist of the fusion of mouse IgG2a Fc region to the N-terminal of Rspo1 with a N-terminal HA epitope tag (Millipore=SCC111).
Genetic integration: Method=Transfection; Gene=MGI; MGI:2183426; Rspo1 (Note=With a N-terminal HA tag).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Caution: Could be identical to HA-R-Spondin1-Fc 293T (Cellosaurus=CVCL_RU08) distributed by AMSBIO. Both cell line product pages cite PubMed=19398967 but the Millipore page states "Cell line is not the published cell line from the Kuo lab, but rather a related cell line developed in the same lab using the reference protocol".
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): Millipore=SCC111

Markers:
AmelogeninX
CSF1PO11,12
D3S135816,17
D5S8187,8,9
D7S82011
D8S117911,12,13,14
D13S31712,14
D16S5399,14
D18S5117,18,19
D21S1128,29,30,30.2
FGA22,23
Penta D9,10
Penta E7,15
TH017,9.3
TPOX11
vWA16,19,20

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Publications

PubMed=19398967; DOI=10.1038/nm.1951; PMCID=PMC2919216
Akifumi Ootani, Xing-Nan Li, Eugenio Sangiorgi, Quoc T. Ho, Hiroo Ueno, Shuji Toda, Hajime Sugihara, Kazuma Fujimoto, Irving Lerner Weissman, Mario Ramberg Capecchi, Calvin Jay Kuo;
Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche.
Nat. Med. 15:701-706(2009)

Cross-references
Cell line collections (Providers) Millipore; SCC111
Encyclopedic resources Wikidata; Q54584138
Entry history
Entry creation05-Mar-2018
Last entry update14-Aug-2025
Version number11