ID   293T R-Spondin1 expressing
AC   CVCL_RU09
SY   R-Spondin1 expressing 293T
DR   Millipore; SCC111
DR   Wikidata; Q54584138
RX   PubMed=19398967;
CC   Characteristics: Expresses mouse Fc-Rspo1 which consist of the fusion of mouse IgG2a Fc region to the N-terminal of Rspo1 with a N-terminal HA epitope tag (Millipore=SCC111).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:2183426; Rspo1 (Note=With a N-terminal HA tag).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Caution: Could be identical to HA-R-Spondin1-Fc 293T (Cellosaurus=CVCL_RU08) distributed by AMSBIO. Both cell line product pages cite PubMed=19398967 but the Millipore page states "Cell line is not the published cell line from the Kuo lab, but rather a related cell line developed in the same lab using the reference protocol".
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
ST   Source(s): Millipore=SCC111
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 12,14
ST   D16S539: 9,14
ST   D18S51: 17,18,19
ST   D21S11: 28,29,30,30.2
ST   D3S1358: 16,17
ST   D5S818: 7,8,9
ST   D7S820: 11
ST   D8S1179: 11,12,13,14
ST   FGA: 22,23
ST   Penta D: 9,10
ST   Penta E: 7,15
ST   TH01: 7,9.3
ST   TPOX: 11
ST   vWA: 16,19,20
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 05-03-18; Last updated: 10-04-25; Version: 10
//
RX   PubMed=19398967; DOI=10.1038/nm.1951; PMCID=PMC2919216;
RA   Ootani A., Li X.-N., Sangiorgi E., Ho Q.T., Ueno H., Toda S.,
RA   Sugihara H., Fujimoto K., Weissman I.L., Capecchi M.R., Kuo C.J.;
RT   "Sustained in vitro intestinal epithelial culture within a
RT   Wnt-dependent stem cell niche.";
RL   Nat. Med. 15:701-706(2009).
//