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Cellosaurus BIONi010-C-15 (CVCL_RM87)

[Text version]
Cell line name BIONi010-C-15
Synonyms BIONi010-C + dox inducible NGN2-GFP
Accession CVCL_RM87
Resource Identification Initiative To cite this cell line use: BIONi010-C-15 (RRID:CVCL_RM87)
Comments From: Bioneer A/S; Horsholm; Denmark.
Population: African.
Characteristics: Expression of NEUROG2/NGN2-eGFP is doxycycline-inducible. Using TALEN NEUROG2-T2A-eGFP under the control of a tetracycline response element and a 2A-Puro was inserted into one allele of the safe harbor locus (AAVS1). At the second locus a 2A-Neo-CAG-rtTA construct was inserted (PubMed=33610017).
Transfected with: HGNC; 13805; NEUROG2.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Derived from site: In situ; Skin, dermis; UBERON=UBERON_0002067.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1E68 (BIONi010-C)
Sex of cell Male
Age at sampling 18Y
Category Induced pluripotent stem cell

PubMed=33610017; DOI=10.1016/j.scr.2021.102240
Schmid B., Holst B., Poulsen U.B., Jorring I., Clausen C., Rasmussen M., Mau-Holzmann U.A., Steeg R., Nuthall H., Ebneth A., Cabrera-Socorro A.
Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus.
Stem Cell Res. 52:102240-102240(2021)

Cell line collections (Providers) EBiSC; BIONi010-C-15
ECACC; 66540570 - Discontinued
Cell line databases/resources hPSCreg; BIONi010-C-15
Biological sample resources BioSamples; SAMEA104008229
Encyclopedic resources Wikidata; Q54796759
Entry history
Entry creation05-Mar-2018
Last entry update30-Jan-2024
Version number11