Cellosaurus Gibco DR4 Mouse Embryonic Fibroblasts, Irradiated (CVCL_RB12)
| Cell line name | Gibco DR4 Mouse Embryonic Fibroblasts, Irradiated |
|---|---|
| Synonyms | Irradiated Gibco DR4 Mouse Embryonic Fibroblasts |
| Accession | CVCL_RB12 |
| Resource Identification Initiative | To cite this cell line use: Gibco DR4 Mouse Embryonic Fibroblasts, Irradiated (RRID:CVCL_RB12) |
| Comments | Characteristics: Used as feeder cells to support the growth of stem cells in the undifferentiated state (Thermo Fisher; A34966). Characteristics: Growth-arrested by irradiation (Thermo Fisher; A34966). Characteristics: Expresses transgenes that make this cell line resistant to four different drugs: geneticin (G418), puromycin, hygromycin and 6-tioguanine (Thermo Fisher; A34966). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCIt; C44386; Gamma radiation. Derived from site: In situ; Embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: DR4 x CF-1. |
| Sex of cell | Mixed sex |
| Age at sampling | 13-14FD |
| Category | Finite cell line |
| Web pages | Provider; Thermo Fisher; A34966; https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017060-DR4-MouseEmbryonicFibroblastsIrradiated-UG.pdf |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q54835825 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 10-Apr-2025 |
| Version number | 10 |