ID   Gibco DR4 Mouse Embryonic Fibroblasts, Irradiated
AC   CVCL_RB12
SY   Irradiated Gibco DR4 Mouse Embryonic Fibroblasts
DR   Wikidata; Q54835825
WW   Provider; Thermo Fisher; A34966; https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017060-DR4-MouseEmbryonicFibroblastsIrradiated-UG.pdf
CC   Characteristics: Used as feeder cells to support the growth of stem cells in the undifferentiated state (Thermo Fisher; A34966).
CC   Characteristics: Growth-arrested by irradiation (Thermo Fisher; A34966).
CC   Characteristics: Expresses transgenes that make this cell line resistant to four different drugs: geneticin (G418), puromycin, hygromycin and 6-tioguanine (Thermo Fisher; A34966).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCIt; C44386; Gamma radiation.
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: DR4 x CF-1.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Mixed sex
AG   13-14FD
CA   Finite cell line
DT   Created: 05-03-18; Last updated: 10-04-25; Version: 10
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