Cellosaurus Mel624-CMV-hNIS-Neo/eGFP-Puro (CVCL_RA31)
| Cell line name | Mel624-CMV-hNIS-Neo/eGFP-Puro |
|---|---|
| Accession | CVCL_RA31 |
| Resource Identification Initiative | To cite this cell line use: Mel624-CMV-hNIS-Neo/eGFP-Puro (RRID:CVCL_RA31) |
| Comments | Characteristics: Reporter cell line, transfected with both the CMV-hNIS-IRES-Neo and LV-eGFP-PGK-Puro vectors. CMV-hNIS-IRES-Neo encodes for human SLC5A5 linked via an internal ribosomal entry site (IRES) to the Neo gene and under control of the CMV promoter. LV-eGFP-PGK-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter. Transfected with: HGNC; 11040; SLC5A5. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: Metastatic; Not specified. |
| Disease | Melanoma (NCIt: C3224) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_8054 (624-mel) |
| Sex of cell | Male |
| Age at sampling | Age unspecified |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL017 |
| Encyclopedic resources | Wikidata; Q54905171 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 29-Jun-2023 |
| Version number | 7 |