ID   Mel624-CMV-hNIS-Neo/eGFP-Puro
AC   CVCL_RA31
DR   Imanis; CL017
DR   Wikidata; Q54905171
CC   Characteristics: Reporter cell line, transduced with both the CMV-hNIS-IRES-Neo and LV-eGFP-PGKp-Puro vectors. CMV-hNIS-IRES-Neo encodes for human SLC5A5 linked via an internal ribosomal entry site (IRES) to the Neo gene and under control of the CMV promoter. LV-eGFP-PGKp-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL017).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11040; SLC5A5.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Discontinued: Imanis; CL017; true.
CC   Derived from site: Metastatic; Not specified.
DI   NCIt; C3224; Melanoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_8054 ! 624-mel
SX   Male
AG   Age unspecified
CA   Cancer cell line
DT   Created: 05-03-18; Last updated: 10-04-25; Version: 11
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