Cellosaurus cell line HT1080-hNIS-Puro (CVCL

Cross-references
Cell line name	HT1080-hNIS-Puro
Accession	CVCL_RA16
Resource Identification Initiative	To cite this cell line use: HT1080-hNIS-Puro (RRID:CVCL_RA16)
Comments	Population: Caucasian. Characteristics: Reporter cell line, transduced with the LV-hNIS-P2A-Puro vector encoding for human SLC5A5 linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL102). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11040; SLC5A5. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations	Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease	Fibrosarcoma (NCIt: C3043) Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0317 (HT-1080)
Sex of cell	Male
Age at sampling	35Y
Category	Cancer cell line
Cell line collections (Providers)	Imanis; CL102
Cell line databases/resources	cancercelllines; CVCL_RA16
Encyclopedic resources	Wikidata; Q54896564
Entry history
Entry creation	05-Mar-2018
Last entry update	14-Aug-2025
Version number	16