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Cellosaurus HT1080-Fluc-Puro (CVCL_RA12)

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Cell line name HT1080-Fluc-Puro
Accession CVCL_RA12
Resource Identification Initiative To cite this cell line use: HT1080-Fluc-Puro (RRID:CVCL_RA12)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-Puro vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL076).
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0317 (HT-1080)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL076
Cell line databases/resources cancercelllines; CVCL_RA12
Encyclopedic resources Wikidata; Q54896560
Entry history
Entry creation05-Mar-2018
Last entry update14-Aug-2025
Version number16