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Cellosaurus CT26.WT-iRFP-Neo (CVCL_RA03)

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Cell line name CT26.WT-iRFP-Neo
Accession CVCL_RA03
Resource Identification Initiative To cite this cell line use: CT26.WT-iRFP-Neo (RRID:CVCL_RA03)
Comments Characteristics: Reporter cell line, transfected with the LV-iRFP-P2A-Neo which encodes for iRFP linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL091).
Genetic integration: Method=Transfection; Gene=FPbase; DGXFA; iRFP (Note=iRFP713, near infra-red fluorescent protein from R.palustris).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: ChEBI; CHEBI:82373; N-nitroso-N-methylurethane (NNMU).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Gene deletion; MGI; MGI:104738; Cdkn2a; Zygosity=Homozygous (from parent cell line).
  • Mutation; MGI; MGI:96680; Kras; Simple; p.Gly12Asp (c.35G>A); Zygosity=Homozygous (from parent cell line).
Disease Mouse colon adenocarcinoma (NCIt: C120044)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_7256 (CT26.WT)
Sex of cell Female
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL091
Encyclopedic resources Wikidata; Q54814715
Entry history
Entry creation05-Mar-2018
Last entry update10-Sep-2024
Version number8