Cellosaurus cell line A375-Fluc-Puro (CVCL

Cellosaurus A375-Fluc-Puro (CVCL_QZ70)

Cross-references
Cell line name	A375-Fluc-Puro
Accession	CVCL_QZ70
Resource Identification Initiative	To cite this cell line use: A375-Fluc-Puro (RRID:CVCL_QZ70)
Comments	Population: Caucasian. Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-Puro vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL055). Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Sequence variations	Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease	Amelanotic melanoma (NCIt: C3802)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0132 (A-375)
Sex of cell	Female
Age at sampling	54Y
Category	Cancer cell line
Cell line collections (Providers)	Imanis; CL055
Cell line databases/resources	cancercelllines; CVCL_QZ70
Encyclopedic resources	Wikidata; Q54606788
Entry history
Entry creation	05-Mar-2018
Last entry update	14-Aug-2025
Version number	16