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Cellosaurus A375-Fluc-Neo/hNIS-Puro (CVCL_QZ69)

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Cell line name A375-Fluc-Neo/hNIS-Puro
Accession CVCL_QZ69
Resource Identification Initiative To cite this cell line use: A375-Fluc-Neo/hNIS-Puro (RRID:CVCL_QZ69)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transfected with both the LV-Fluc-P2A-Neo and LV-hNIS-P2A-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-hNIS-P2A-Puro encodes for human SLC5A5 linked via a P2A cleavage peptide to the pac gene and under control of the spleen focus-forming virus (SFFV) promoter.
Transfected with: HGNC; 11040; SLC5A5.
Transfected with: UniProtKB; P08659; Firefly luciferase.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0132 (A-375)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL087
Cell line databases/resources cancercelllines; CVCL_QZ69
Encyclopedic resources Wikidata; Q54606785
Entry history
Entry creation05-Mar-2018
Last entry update05-Oct-2023
Version number13