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Cellosaurus HepAD38 (CVCL_M177)

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Cell line name HepAD38
Synonyms Hep AD38; HepG2 AD38; HepG2-AD38; Hep G2 AD 38; AD-38; AD38
Accession CVCL_M177
Resource Identification Initiative To cite this cell line use: HepAD38 (RRID:CVCL_M177)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12077.
Population: Caucasian.
Biotechnology: Used in a bioassay that permits the large-scale screening of compound libraries for inhibitors of HBV replication.
Virology: Inducibly expresses a hepatitis B virus (HBV) genome under the control of a tet operator/CMV promoter. Upon removal of tetracycline the cell line produces viral RNA and secrete virus-like particles into the supernatant.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0027 (Hep-G2)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-3561

Markers:
AmelogeninX,Y
CSF1PO10,11
D5S81811,12
D7S82010
D13S3179,13
D16S53912,13
TH019
TPOX8,9
vWA17

Run an STR similarity search on this cell line
Publications

PubMed=9257747; DOI=10.1128/AAC.41.8.1715; PMCID=PMC163991
Stephanie K. Ladner, Michael J. Otto, Christopher S. Barker, Katie Zaifert, Guang-Hua Wang, Ju-Tao Guo, Christoph Seeger, Robert W. King;
Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: a novel system for screening potential inhibitors of HBV replication.
Antimicrob. Agents Chemother. 41:1715-1720(1997)

Patent=US5723319
Robert W. King, Christopher S. Barker, Christoph Seeger;
Cultured cell line that inducibly expresses the hepatitis B virus genome, and uses thereof for screening antiviral substances.
Patent number US5723319, 03-Mar-1998

PubMed=9875378
Stephanie K. Ladner, Timothy J. Miller, Michael J. Otto, Robert W. King;
The hepatitis B virus M539V polymerase variation responsible for 3TC resistance also confers cross-resistance to other nucleoside analogues.
Antivir. Chem. Chemother. 9:65-72(1998)

PubMed=21331898; DOI=10.1385/1-59259-245-7:43
Robert W. King, Stephanie K. Ladner;
Hep AD38 assay: a high-throughput, cell-based screen for the evaluation of compounds against hepatitis B virus.
Methods Mol. Med. 24:43-50(2000)

Cross-references
Cell line collections (Providers) ATCC; CRL-3561
ATCC; CRL-12077 - Discontinued
Cell line databases/resources cancercelllines; CVCL_M177
Anatomy/cell type resources BTO; BTO_0006298
Encyclopedic resources Wikidata; Q54882829
Entry history
Entry creation06-May-2013
Last entry update19-Dec-2024
Version number23