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Cellosaurus MLO-Y4 (CVCL_M098)

[Text version]
Cell line name MLO-Y4
Synonyms MLOY4; Murine Long bone Osteocyte-Y4
Accession CVCL_M098
Resource Identification Initiative To cite this cell line use: MLO-Y4 (RRID:CVCL_M098)
Comments Group: Patented cell line.
Group: Space-flown cell line (cellonaut).
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12161.
Characteristics: Useful to study effects of fluid flow and substrate stretching on activation of osteocyte-specific signaling pathways (Kerafast=EKC002).
Doubling time: 15.8 hours (CelloPub=CLPUB00625).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Bone, long bone; UBERON=UBERON_0002495.
Cell type: Osteocyte; CL=CL_0000137.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: (C57BL/6J x BALB/cJ) F2 Bgla promoter T-Ag transgenic.
Sex of cell Sex unspecified
Age at sampling 14D
Category Transformed cell line

PubMed=9421234; DOI=10.1359/jbmr.1997.12.12.2014
Kato Y., Windle J.J., Koop B.A., Mundy G.R., Bonewald L.F.
Establishment of an osteocyte-like cell line, MLO-Y4.
J. Bone Miner. Res. 12:2014-2023(1997)

PubMed=10084404; DOI=10.1007/s007740050066
Bonewald L.F.
Establishment and characterization of an osteocyte-like cell line, MLO-Y4.
J. Bone Miner. Metab. 17:61-65(1999)

Bonewald L.F., Kato Y.
Osteocyte cell lines.
Patent number US6358737, 19-Mar-2002

PubMed=12412815; DOI=10.1359/jbmr.2002.17.11.2068
Zhao S., Zhang Y.K., Harris S., Ahuja S.S., Bonewald L.F.
MLO-Y4 osteocyte-like cells support osteoclast formation and activation.
J. Bone Miner. Res. 17:2068-2079(2002)

Lau P., Hellweg C.E., Kirchner S., Arenz A., Baumstark-Khan C., Horneck G.
Induction of DNA-strand breaks after X-irradiation in murine bone cells of various differentiation capacities.
(In) Proceedings of 35th COSPAR Scientific Assembly; pp.1960-1960; Paris (2004)

PubMed=16738320; DOI=10.1128/mcb.02120-05
Zhang K.-Q., Barragan-Adjemian C., Ye L., Kotha S., Dallas M., Lu Y.-B., Zhao S.-J., Harris M., Harris S.E., Feng J.Q., Bonewald L.F.
E11/gp38 selective expression in osteocytes: regulation by mechanical strain and role in dendrite elongation.
Mol. Cell. Biol. 26:4539-4552(2006)

PubMed=22130923; DOI=10.1007/978-1-61779-415-5_6
Rosser J., Bonewald L.F.
Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5.
Methods Mol. Biol. 816:67-81(2012)

Cell line collections (Providers) Kerafast; EKC002
Cell line databases/resources Lonza; 166
Anatomy/cell type resources BTO; BTO:0005489
Encyclopedic resources Wikidata; Q54905979
Experimental variables resources EFO; EFO_0005394
Gene expression databases GEO; GSM1520095
GEO; GSM1520096
GEO; GSM1520097
GEO; GSM1520098
GEO; GSM1520099
GEO; GSM1520100
GEO; GSM1520101
GEO; GSM1520102
GEO; GSM1520103
GEO; GSM1520104
GEO; GSM1520105
GEO; GSM1520106
Entry history
Entry creation06-May-2013
Last entry update29-Jun-2023
Version number20