Cellosaurus cell line MLO-Y4 (CVCL

Cellosaurus MLO-Y4 (CVCL_M098)

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Cell line name

MLO-Y4

Synonyms

MLOY4; Murine Long bone Osteocyte-Y4

Accession

CVCL_M098

Resource Identification Initiative

To cite this cell line use: MLO-Y4 (RRID:CVCL_M098)

Comments

Group: Patented cell line.
Group: Space-flown cell line (cellonaut).
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12161.
Characteristics: Useful to study effects of fluid flow and substrate stretching on activation of osteocyte-specific signaling pathways (Kerafast=EKC002).
Doubling time: 15.8 hours (CelloPub=CLPUB00625).
Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Miscellaneous: STR profile from personal communication of Yi, Lan.
Derived from site: In situ; Bone, long bone; UBERON=UBERON_0002495.
Cell type: Osteocyte; CL=CL_0000137.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: (C57BL/6J x BALB/cJ) F2 Bgla promoter T-Ag transgenic.

Hierarchy

Children:

CVCL_C8UU (MLO-Y4-Luc)

CVCL_E0Z7 (Ubigene MLO-Y4 Tnfsf11 KO)

Sex of cell

Sex unspecified

Age at sampling

14D

Category

Transformed cell line

STR profile

Source(s): Direct_author_submission; Ubigene=YC-C037

Markers:

Mouse STR 1-1	16
Mouse STR 1-2	17,18
Mouse STR 2-1	16
Mouse STR 3-2	12,13
Mouse STR 4-2	20.3,21.3 (Ubigene=YC-C037) 21.3 (Direct_author_submission)
Mouse STR 5-5	14
Mouse STR 6-4	18
Mouse STR 6-7	12 (Direct_author_submission) 17 (Ubigene=YC-C037)
Mouse STR 7-1	29
Mouse STR 8-1	13,14
Mouse STR 11-2	18
Mouse STR 12-1	17
Mouse STR 13-1	16.2,17.2
Mouse STR 15-3	22.3
Mouse STR 17-2	16,17
Mouse STR 18-3	17,18 (Ubigene=YC-C037) 18 (Direct_author_submission)
Mouse STR 19-2	13
Mouse STR X-1	25

Publications

PubMed=9421234; DOI=10.1359/jbmr.1997.12.12.2014
Yoichi Kato, Jolene J. Windle, Barbara A. Koop, Gregory Robert Mundy, Lynda Faye Bonewald;
Establishment of an osteocyte-like cell line, MLO-Y4.
J. Bone Miner. Res. 12:2014-2023(1997)

PubMed=10084404; DOI=10.1007/s007740050066
Lynda Faye Bonewald;
Establishment and characterization of an osteocyte-like cell line, MLO-Y4.
J. Bone Miner. Metab. 17:61-65(1999)

Patent=US6358737
Lynda Faye Bonewald, Yoichi Kato;
Osteocyte cell lines.
Patent number US6358737, 19-Mar-2002

PubMed=12412815; DOI=10.1359/jbmr.2002.17.11.2068
Shu-Jie Zhao, Yoichi Kato, Y. Zhang, Stephen E. Harris, Seema Singh Ahuja, Lynda Faye Bonewald;
MLO-Y4 osteocyte-like cells support osteoclast formation and activation.
J. Bone Miner. Res. 17:2068-2079(2002)

CLPUB00625
Patrick Lau, Christine Elisabeth Hellweg, Stefan Kirchner, Andrea Arenz, Christa Baumstark-Khan, Gerda Horneck;
Induction of DNA-strand breaks after X-irradiation in murine bone cells of various differentiation capacities.
(In conference) Abstracts of the 35th COSPAR scientific assembly; pp.1960-1960; Committee on Space Research; Paris; France (2004)

PubMed=16738320; DOI=10.1128/mcb.02120-05; PMCID=PMC1489126
Ke-Qin Zhang, Cielo Barragan-Adjemian, Ling Ye, Shiva Kotha, Mark Dallas, Yong-Bo Lu, Shu-Jie Zhao, Marie Harris, Stephen E. Harris, Jian Q. Feng, Lynda Faye Bonewald;
E11/gp38 selective expression in osteocytes: regulation by mechanical strain and role in dendrite elongation.
Mol. Cell. Biol. 26:4539-4552(2006)

PubMed=22130923; DOI=10.1007/978-1-61779-415-5_6
Jennifer Rosser, Lynda Faye Bonewald;
Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5.
Methods Mol. Biol. 816:67-81(2012)

Cross-references

Cell line collections (Providers)

Kerafast; EKC002
Ubigene; YC-C037

Cell line databases/resources

Lonza; 166

Anatomy/cell type resources

BTO; BTO_0005489

Encyclopedic resources

Wikidata; Q54905979

Experimental variables resources

EFO; EFO_0005394

Gene expression databases