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Cellosaurus MN9D (CVCL_M067)

[Text version]
Cell line name MN9D
Synonyms MN-9D
Accession CVCL_M067
Resource Identification Initiative To cite this cell line use: MN9D (RRID:CVCL_M067)
Comments Characteristics: Extensively used to model dopaminergic neurons and to test mechanisms and potential therapeutics relevant to the loss of DA neurons in Parkinson's disease (Millipore=SCC281).
Characteristics: Fusion of N18TG2 with rostral mesencephalic neurons from a 14-day-old C57BL/6J mouse embryo (PubMed=1913182).
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_2132 (N18TG2)
Category Hybrid cell line
STR profile Source(s): Millipore=SCC281

Markers:
Mouse STR 1-111,17
Mouse STR 1-217,19
Mouse STR 2-116
Mouse STR 3-213,14
Mouse STR 4-220.3,22.3
Mouse STR 5-515,17
Mouse STR 6-418,20
Mouse STR 6-712,17
Mouse STR 7-125.2
Mouse STR 8-116,17
Mouse STR 11-215,16
Mouse STR 12-116,17
Mouse STR 13-116.2,17.2,18.2
Mouse STR 15-322.3,23.3
Mouse STR 17-215,16
Mouse STR 18-316,22
Mouse STR 19-212,13
Mouse STR X-125,28,29

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Publications

PubMed=1913182; DOI=10.1016/0006-8993(91)90661-E
Hyung K. Choi, Lisa A. Won, Paul J. Kontur, David N. Hammond, Aaron P. Fox, Bruce H. Wainer, Philip C. Hoffmann, Alfred Heller;
Immortalization of embryonic mesencephalic dopaminergic neurons by somatic cell fusion.
Brain Res. 552:67-76(1991)

PubMed=1357658; DOI=10.1073/pnas.89.19.8943; PMCID=PMC50040
Hyung K. Choi, Lisa A. Won, John D. Roback, Bruce H. Wainer, Alfred Heller;
Specific modulation of dopamine expression in neuronal hybrid cells by primary cells from different brain regions.
Proc. Natl. Acad. Sci. U.S.A. 89:8943-8947(1992)

PubMed=17028430; DOI=10.1159/000095115
Caroline E. Rick, Allison D. Ebert, Tamas Virag, Martha C. Bohn, Dalton James Surmeier;
Differentiated dopaminergic MN9D cells only partially recapitulate the electrophysiological properties of midbrain dopaminergic neurons.
Dev. Neurosci. 28:528-537(2006)

Cross-references
Cell line collections (Providers) Millipore; SCC281
Cell line databases/resources Lonza; 979
Anatomy/cell type resources BTO; BTO_0003945
Encyclopedic resources Wikidata; Q54906266
Experimental variables resources EFO; EFO_0006455
Entry history
Entry creation06-May-2013
Last entry update10-Apr-2025
Version number13